Table 3.
Parameters that were measured to evaluate their impact on the occurrence of high serum indices in clear serum samples; methods, measuring ranges, instruments and reagent manufacturers.
| Analyte | Instrument | Method | Measuring range | Manufacturer |
|---|---|---|---|---|
| IgG | Siemens BN II nephelometer | Nephelometric method | 1.4–64.0 g/L1 (1/400 dilution) | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| IgA | Siemens BN II nephelometer | Siemens BN II nephelometer Nephelometric method | Siemens BN II nephelometer Nephelometric method 0.23–8.00 g/L2 (1/20 dilution) | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| IgM | IgM Siemens BN II nephelometer | IgM Siemens BN II nephelometer Nephelometric method | 0.18–6.40 g/L2 (1/20 dilution) | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| Kappa light chains | Kappa light chains Siemens BN II nephelometer | Nephelometric method | 0.28–9.10 g/L1 (1/20 dilution) | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| Lambda light chains | Siemens BN II nephelometer | Nephelometric method | 0.16–5.00 g/L1 (1/20 dilution) | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| Monoclonal immunoglobulin | Monoclonal immunoglobulin Interlab Microtech 648 PC | Monoclonal immunoglobulin Interlab Microtech 648 PC Serum protein electrophoresis | / 3 | Interlab Scientific Instruments, Roma, Italy |
| Monoclonal immunoglobulin | Sebia Hydrasis | Immuno-electrophoresis | / 4 | Sebia, Lisses, Cedex, France |
| Total cholesterol | Total cholesterol Siemens Dimension RXL Max, Vista | Cholesterol esterase | 1.30–15.50 mmol/L2 | Siemens HealthCare Diagnostics, Newark, DE, USA |
| Triglycerides | Siemens Dimension RXL Max, Vista | GPO-PAP Enzymatic | 0.02–11.30 mmol/L2 | Siemens HealthCare Diagnostics, Newark, DE, USA |
| HDL cholesterol | Siemens Dimension RXL Max, Vista | Homogenous direct (enzymatic colorimetric) method | 0.05–3.89 mmol/L2 | Siemens HealthCare Diagnostics, Newark, DE, USA |
| LDL cholesterol | Siemens Dimension RXL Max, Vista | Direct (enzymatic colorimetric) method | 0.00–7.80 mmol/L2 | Siemens HealthCare Diagnostics, Newark, DE, USA |
| Total protein (TP) | Siemens Dimension RXL Max, Vista | Photometric colour test (biuret) | 0–120 g/L2 | Siemens HealthCare Diagnostics, Newark, DE, USA |
| Lp(a) | Siemens BN ProSpec nephelometer | Nephelometric method | 0.10–1.60 g/L1 | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| ApoA1 | Siemens BN II nephelometer | Nephelometric method | 0.19–6.00 g/L1 | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| ApoB | Siemens BN II nephelometer | Nephelometric method | 0.25–4.00 g/L2 | Siemens HealthCare Diagnostics Products GmbH, Marburg, Germany |
| Rheumatoid factor (RF) | Siemens BN ProSpec nephelometer | Nephelometric method | 10–600 IU/mL2 | Siemens HealthCare Diagnostics Products GmbH, Marburg, |
| Cryoglobulins | PU 8675 VIS Spectrophotometer | Lowry method | 0–400 mg/L | Germany Philips, Cambridge, United Kingdom |
| Serum index | Olympus AU640 | LIH | / | Olympus Diagnostica GmbH, Lismeehan, Ireland5 |
| Serum index | Hitachi 911 | LIH | / | Roche Diagnostics GmbH, Mannheim, Germany |
| Serum index | Siemens Dimension RXL Max, Vista | HIL | / | Siemens HealthCare Diagnostics, Newark, DE, USA |
1
A change of lower and upper range value is possible with dilution of the sample.
2
A change of upper range value is possible with dilution of the sample.
3
Detection limit for monoclonal immunoglobulin is 1.0 g/L.
4
Detection limit for monoclonal immunoglobulin is between 0.12–0.25 g/L.
5
Name regarding the time of measurements; otherwise Beckman Coulter.