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. 2013 Oct 9;23(4):1002–1012. doi: 10.1093/hmg/ddt496

Figure 3.

Figure 3.

Destabilization of cone PDE6 and RetGC1 in animals lacking Aipl1. (A) Immunoblotting of retinal extracts from Nrl−/− Aipl1−/− compared with Nrl−/− Aipl1+/− mice at P12 with antibodies against indicated proteins. Equal amounts (150 µg) of total proteins were loaded in each lane. As expected, Aipl1 was not observed in Nrl−/− Aipl1−/− double mutant mice. Tubulin serves as a loading control. Protein molecular weight marker is shown on the left. (B) Quantitation of cone PDE6, RetGC1 and GαT2 levels in retinal extracts from Nrl−/− Aipl1+/−and Nrl−/− Aipl1−/− double knockout animals. Band densities were measured in LI-COR odyssey and normalized to levels present in Nrl−/− Aipl1+/−animals. Each column shown is the mean of normalized values obtained from three independent samples. *P < 0.001, Nrl−/− Aipl1+/− versus Nrl−/− Aipl1−/− double knockout mice by Student's t-test. (C) RT-PCR results showing message levels of RetGC1 (Gucy2e) and cone PDE6α′ (Pde6c).