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. 2013 Oct 9;23(4):1002–1012. doi: 10.1093/hmg/ddt496

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Figure 5. — Assembly and stability of cone PDE6 is dependent on Aipl1. (A) Protein synthesis was monitored in retinas isolated from Nrl^−/−Aipl1^+/− or mice lacking Aipl1 (Nrl^−/− Aipl1^−/−). The retinas were labeled with radioactive methionine for the indicated amount of time. The amount of proteins synthesized was measured by IP using antibodies that recognize Aipl1, PDE6α′, PDE6γ′ or cone transducin (GαT2) followed by fluorography. (B) Stability of the synthesized protein was tested after 1.5 h of radioactive labeling followed by indicated 0–3 h of chase using non-radioactive methionine. GαT2 serves as a control. (C) PDE6 assembly verified after 1.5 h of radioactive labeling followed by IP with ROS-I antibody and fluorography. For all experiments in this panel, retinas from P12 animals were used.