Figure 6.

Aipl1 contributes to association of PDE6 with membranes. Isotonic protein extraction examining the association of proteins with membranes. (A) After sub-cellular fractionation of retinal extracts, total (T), soluble cytosolic fraction (C) and re-suspended membrane fraction (M) were analyzed by western blotting with indicated antibodies. (B) Protein density in each fraction was measured using Li-COR Odyssey and normalized to protein density found in total fraction. Cone PDE6 and RetGC1 values shown are the mean of normalized measurements obtained from three independent samples. *P < 0.01, Nrl^−/− Aipl1^+/− versus Nrl^−/− Aipl1^−/− double knockout mice by Student's t-test.