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. 2013 Oct 8;23(4):1036–1044. doi: 10.1093/hmg/ddt499

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Figure 3. — FLIM analysis of MeCP2 interaction with HP1α in STHdh cell lines. Fluorescent imaging of the donor fluorophore (Alexa488) in HP1α/Alexa488 and MeCP2/Cy3 immunostaining (A, C and E). Fluorescent imaging of the donor fluorophore with fluorescent lifetimes pseudocolored (see color scale) and mapped on the image pixel-by-pixel (B, D and F). (A and B) FLIM analysis of the donor fluorophore (HP1α/Alexa488) in the no acceptor condition, STHdh^7/7 cells. (C and D) FLIM analysis of HP1α/Alexa488 immunostaining of STHdh^7/7 cells in the presence of MeCP2/Cy3, acceptor fluorophore. (E and F) FLIM analysis of HP1α/Alexa488 immunostaining of STHdh^111/111cells in the presence of MeCP2/Cy3, acceptor fluorophore. (G) Average fluorescent lifetime of the HP1α/Alexa488 donor fluorophore in STHdh^7/7 cells in the absence of acceptor fluorophore (Alexa488 alone, black bars) and in the presence of MeCP2/Cy3 acceptor fluorophore in STHdh^7/7 (blue bars) and STHdh^111/111cells (red bars).