(A-D) Detection of autoreactive IgM-isotype (A), IgG-isotype (B) and pathogenic class-switched IgG anti-dsDNA (C) or anti-sm/RNP (D) antibodies in the serum of 8- month-old control, B-lyn−/− and B-lyn−/− myd88−/− mice. Bars represent mean ± SEM from 6 mice per group. * P ≤ 0.05, ** P ≤ 0.01, ** P ≤ 0.001 (One-way ANOVA).
(E) H&E, C3 and IgG staining of kidney sections from 8 month-old B-lyn−/− myd88−/− mice. Representative pictures are shown. Scale bars represent 50 μm.