Skip to main content
. Author manuscript; available in PMC: 2014 Jan 23.
Published in final edited form as: Methods Mol Biol. 2009;505:187–204. doi: 10.1007/978-1-60327-575-0_11

Fig. 2.

Fig. 2

Effect of knocking down NM23-H1 on protein levels. MCF-7 cells were transfected with control or NM23-H1 siRNA for 96 h. Cells were then treated with ethanol vehicle or 17β-estradiol (E2) for 24 h, lysed, and subjected to Western blot analysis with an antibody that recognizes NM23-H1 and NM23-H2, ERα, the progesterone receptors PR-A and PR-B, or GAPDH.