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. Author manuscript; available in PMC: 2014 Jan 23.
Published in final edited form as: Methods Mol Biol. 2009;505:187–204. doi: 10.1007/978-1-60327-575-0_11

Fig. 3.

Fig. 3

Effect of decreased NM23-H1 on cellular mRNA levels. MCF-7 cells were transfected with control or NM23-H1 siRNA for 96 h, treated with ethanol vehicle (light gray bars) or E2 (dark gray bars) for 24 h, and lysed. RNA was isolated, cDNA was synthesized, and qRT-PCR was carried out with gene-specific primers. Relative fold change was determined using the comparative Ct method and the housekeeping gene, 36B4, as the internal control. Data from one experiment, which had been done in triplicate, is presented as the mean ± SD.