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. 2014 Jan 23;9(1):e84707. doi: 10.1371/journal.pone.0084707

Figure 4. Splenocyte IFN-γ ELISpot responses to specific vaccine antigen derived peptide pools.

Figure 4

Cells isolated from mouse spleens at termination were stimulated with peptide pools (15 mers overlapping by 11 aa) that covered the entire coding sequence of the Gag and Env protein (two pools for each peptide) that were expressed from the transgenes present in the two plasmid DNAs and the MVA-C vector. (A) Specific peptide pool responses of splenocytes from all study groups. (B) Comparison of splenocyte IFN-γ ELISpot responses to the Env and the Gag peptide pools in animals that had been DNA primed then inoculated with either MVA-C and CN54gp140+GLA sequentially or with the MVA and protein or with all three boost vaccine components concurrently. Responses from each group of animals are shown as group mean spot forming units (SFU)/million splenocytes +/− SEM. Groups were statistically compared using a one-way Anova (*p<0.05; **p<0.01; ***p<0.005; **** p<0.001).