Figure 5. Polyfunctional cytokine responses of peptide pool stimulated splenocytes.

Cells from each animal were stimulated with each different peptide pool for 6°C in the presence of a golgi secretion inhibitor. CD4+ or CD8+ T cell populations were gated and single, double or triple cytokine expressing cell percentage enumerated. The percentage cytokine expression of each animal was averaged within the group and is expressed as a pie-chart of relative proportions of the total cytokine expressing cell population. Pie slice colour key indicates the particular cytokine(s) expressed.