Figure 3. Assay for the detection of L925I mutation in individual bugs. The mutation suppresses a cleavage site for Sac I restriction endonuclease.

As a result, when the susceptible allele (S) is present, two fragments are observed (51 bp and 426 bp). When the resistant allele is present (R), a single fragment of 477 bp is observed. M indicates the position of the standard size marker, in which the lower five bands are 100, 200, 300, 400 and 500 bp.