Table 5.
Rescue of the βftz-f1ex7hs-βFTZ-F1 null mutant larvae by targeted βFTZ-F1 expression in Inka cells.
| Total number of larvae used after hs of embryos | Number and % of rescued larvae after heat or RU486 treatment | ||||
|---|---|---|---|---|---|
|
| |||||
| 1st instar | 1st instar | 2nd instar | rescued | ||
| ETHGS/UAS-βFTZ-F1; ftz-f1ex7hs-βFTZ-F1 | |||||
| hs | 59 | hs | 11 | 48 | 81% |
| hs | 67 | hs | 14 | 53 | 79% |
| hs | 56 | RU486 | 11 | 45 | 80% |
| hs | 59 | RU486 | 15 | 44 | 75% |
| hs | 90 | RU486 | 22 | 68 | 76% |
|
| |||||
| ftz-f1ex7hs-βFTZ-F1 | |||||
| hs | 87 | hs | 18 | 69 | 79% |
| hs | 75 | RU486 | 75 | 0 | 0% |
Embryos were rescued by heat shock (hs) during 13-16 hours AEL. Surviving 1st instar larvae were heat treated or fed RU486 treated during 42-46 hours AEL. The number of 1st or 2nd instar larvae was counted during 54-60 hours AEL.