Fig. 5.

Comparative transcriptomics reveals the importance of polysaccharide esterases and oligosaccharide hydrolases. (A) Three-way comparison of the regulons for pectin, xylan and cellulose (Coradetti et al., 2012). A regulon is comprised of all genes with elevated expression levels on a given C-source vs. both control conditions: NoC (starved) and sucrose (glucose-repressed) (see also Fig. S5 and Supporting data set 3). (B) Functional categorization of the genes in all seven Venn-diagram fractions resulting from the three-way comparison. The relative contribution of a functional group to each pool is depicted (total number of genes in each pool = 100%). Carbohydrate-active properties (glycoside hydrolases (GH), dark blue; polysaccharide lyases (PL), red; and carbohydrate esterases (CE), green) are clearly enriched in the four intersections. In particular the central section (29 genes induced under all conditions) is enriched for CEs and GHs acting as oligosaccharide hydrolases. Pe: pectin; Av: Avicel; Xy: xylan; ∩: intersection between sets.