Fig. 7.

Screening of strains containing gene deletions for secreted proteins with pectin-specific expression patterns. N. crassa strains containing deletions of genes present in pectin-specific clusters 6 and 10 (Fig. 6) and that were also identified in the pectin and OPP secretomes (see Fig. S7 for annotation) were assayed for their phenotype when grown on pectin as sole C-source for four days. The fungal dry weight, the concentration of secreted protein, and the consumed pectin (the difference between day 0 and day 4 in total reducing ends in the culture supernatants) were measured and compared to WT (FGSC# 2489), which was set to 100%. The strains were ordered according to their recorded dry weight from the least (left) to the most (right). Bars represent standard deviations. (*) indicates a significant difference from WT with an unadjusted P-value of < 0.003 using a one-way Anova.