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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Mol Microbiol. 2013 Dec 4;91(2):275–299. doi: 10.1111/mmi.12459

Fig. 8.

Fig. 8

Growth on Avicel triggers the unfolded protein response (UPR). (A) Localization of the unconventional IRE-1-dependent intron in the hac-1 mRNA. Shown is only the sequence in the immediate vicinity of the 23 nt intron including its translation, thereby showing the frame shift resulting from the splicing. (B) Relative quantification of the amount of hac-1 mRNA splice variants by qPCR. Primers were chosen such that “total” hac-1 transcript could be quantified (primers #1 and #2 in the above diagram) as well as the spliced (“ON”; primers #3 and #5), or non-spliced (“OFF”; primers #3 and #4) versions individually. For quantitation, 16h sucrose grown cultures were transferred to either fresh 2% sucrose, 2% sucrose + 10 mM DTT, or 0.5% Avicel. After 4h RNA was harvested, purified and subjected to qPCR. The results are given as fold-induction over the expression state on 2% sucrose at 4h for each target.