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. 2014 Jan 23;9(1):e85452. doi: 10.1371/journal.pone.0085452

Figure 6. HIV-1 LTR Transactivation by Tat C variants and B/C Tat.

Figure 6

HEK293 cells were co-transfected with 200 ng Tat variants (TatN12, TatD60 and TatVT6) and 50 ng of pGL3-Luc vector containing subtype B or C LTR, and pGL3-Luc B or C LTR alone was used as control. After 24 hours of transfection, cells were lysed with lysis buffer and the luciferase activity was measured in a luminometer. The relative transactivation was expressed as mean luciferase units obtained from luciferase assay. The error bar represents the standard deviation of luciferase units and the p values less than 0.05 represents the significance of luciferase activity.