Figure 10. Summary of immune response in peripheral blood of ICR mice with different clinical courses of PyL infection.

Left: ED mice on day 6 pi reached high parasitemia, accompanied by a large increase of CD4⁺CD25⁺ cells, immature T1 B cells, innate response cells an overproduction of cytokines. Right: S mice on day 6 pi showed low parasitemia with a slight increase in the circulation of mature and immature B cells and a limited production of cytokines. On day 14 pi, S mice reached around 45% parasitemia and increased the number of activated B and CD4⁺ T cells in addition to high levels of circulating anti-parasite specific IgGs. Parasitemia clearance on day 21 pi was concurrent with the highest leukocytosis level, while increase in specific humoral response continued. In S mice the major increases of leukocyte populations in comparison to healthy mice were in activated CD4⁺ and CD8⁺ T cells, class-switched B cells and, to a lesser extent, T1 B cells and CD4⁺CD25⁺ cells.