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. 2014 Jan 23;9(1):e86034. doi: 10.1371/journal.pone.0086034

Figure 3. Inhibitory effect of PEP-1-PON1 protein on LPS-induced inflammatory response in Raw 264.7 cells.

Figure 3

Raw 264.7 cells were stimulated with LPS with or without pretreatment with PEP-1-PON1 protein for 1 h. Cell lysates (A: 10 ng/ml LPS for 24 h, B: 1 µg/ml LPS for 12 h) were prepared and analyzed for COX-2 and iNOS protein expression by Western blotting and band intensity by densitometer (a). After total RNA was extracted from the cells (10 ng/ml and 1 µg/ml LPS for 24 h), cytokines (IL-1β, L-6, TNF-α) and β-actin mRNA were analyzed by RT-PCR using specific primers and band intensity by densitometer (b). *P<0.01, compared with LPS treated cells.