Skip to main content
. 2014 Jan 23;9(1):e86034. doi: 10.1371/journal.pone.0086034

Figure 4. Inhibitory effect of PEP-1-PON1 on LPS-induced MAPK and NF-kB activations.

Figure 4

Raw 264.7 cells were stimulated with 1 µg/ml LPS for 15 min with or without pretreatment with PEP-1-PON1 protein for 1 h. Cells extract prepared and analyzed for MAPK protein activation by Western blotting and band intensity by densitometer (A). Phosphorylation and the degradation of p65 and IkBα were analyzed by Western blotting and band intensity by densitometer (B). ‘p’ indicates the phosphorylated form of the protein. *P<0.01, compared with LPS treated cells.