Figure 6. Effect of transduction of PEP-1-PON1 proteins against H₂O₂-induced cell viability and DNA fragmentation.

H₂O₂ (1 mM and 1.5 mM, 16 h) was added to Raw 264.7 cells pretreated with PEP-1-PON1 (0.3 µM) for 1 h. Cell viabilities were estimated by with a colorimetric assay using MTT (A). **P<0.01, compared with H₂O₂ treated cells. Cells were treated with PEP-1-PON1 protein (0.3 µM) for 1 h, and then exposed to H₂O₂ (1 mM for 15 h and 5 mM for 4 h). DNA fragmentation was detected by TUNEL staining (B). Scale bar = 50 µm.