Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 20;9:259. doi: 10.1186/1746-6148-9-259

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 van Beusekom et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

ATP-dependent transport of TCA, CA and GCA in membrane vesicles (7.5 μg protein) expressing BSEP/Bsep of human (▲), dogs (□), and cats (○). Vesicles were incubated in 10 mMTris Base buffer (pH 7.4) containing 250 mM Sucrose, 10 mM MgCl₂ and 4 mM ATP or AMP, at 37°C for 5 min (TCA) or 7.5 min (CA and GCA). ATP-dependent transport was calculated by subtracting transport in presence of AMP from that in presence of ATP. Curves were fitted by GraphPad Prism 6.01 software (San Diego, California, USA) and V_max was calculated. Measurements were performed in duplicate in at least three independent experiments. The mean of the duplicates of each experiment was transformed as a relative activity to the V_max (=100%). Each value in the graph is the mean ± SD of the relative activities of three independent experiments.