Table 2.
Km values for TCA, GCA and CA of human, dog and cat BSEP/Bsep containing membrane vesicles (7.5 μg protein)
|
Km (μM) |
|||
|---|---|---|---|
| Human | Dog | Cat | |
| TCA |
4.1 ± 0.5 |
3.4 ± 0.3 |
7.5 ± 0.7 |
| GCA |
9.0 ± 2.0 |
19.3 ± 3.5 |
13.4 ± 2.0 |
| CA | 11.3 ± 4.3 | 27.2 ± 3.2a | 41.2 ± 11.3b,c |
asignificantly different from TCA in dogs.
bsignificantly different from TCA in cats.
csignificantly different from GCA in cats.
Km/Vmax curves were fitted by GraphPad Prism 6.01 software (San Diego, California, USA) and Km was calculated. Measurements were performed in duplicate in at least three independent experiments and mean Km ± SD are given in the table. Statistical significance was determined by a two-way ANOVA followed by the Bonferroni post-hoc test. P < 0.05 indicates a significant difference.