Figure 4.

Effects of DHT on activation of MAPK pathway in LPS-stimulated RAW264.7 macrophage. RAW264.7 macrophage were pre-treated with DHT for 30 min, followed by stimulating with LPS (100 ng/ml) for 30 min. (A) The phosphorylation and total protein levels of MAPK (ERK1/2, JNK1/2 and p38) were determined by Western blot analysis. (B) Quantification of the level of phosphorylation measured by the densitometry scanning. The data was expressed as RI (relative intensity) and the means ± SE from three separated experiments. *P < 0.05, **P < 0.01, significantly with the LPS-treated group only.