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. 2014 Jan 23;9(1):e86102. doi: 10.1371/journal.pone.0086102

Figure 2. Inhibit GLUT-1 transcription by FIH-I in U87 under hypoxia GLUT-1 expression was inhibited by PTEN under normoxic condition.

Figure 2

U87 cells were transfected with FIH-1 and PTEN expression plasmids either alone or in combination and cultured in both hypoxic and normoxic conditions. 2A: Total RNA was obtained and followed with RQ-PCR using primers specific for GLUT-1 and 36B4 (internal control). A significant reduction of GLUT-1 mRNA levels was observed in cells expressing PTEN, FIH-1, or both under normoxic conditions, but interestingly, GLUT-1 mRNA levels were not changed in cells expressing PTEN under hypoxic conditions. Average and standard deviation of three independent experiments were calculated. * Represents p value less than 0.05. 2B: Whole cell lysates from each experimental condition were collected and protein levels of FIH-1 (top panel) and PTEN (middle panel) were examined by Western blot. β-actin was used as a loading control (lower panel).