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. 2014 Jan 23;9(1):e86358. doi: 10.1371/journal.pone.0086358

Figure 4. Ku70 and Ku80 bind to AP sites.

Figure 4

We show 10% input for in vitro translated product. The same concentration of biotinylated substrate were used for each reaction of the binding assays. Right panel shows the relative band intensity as measured with the Kodak document program and as compared to 10% input. Shown is the average of three experiments with error bars (standard deviation). Statistics are shown in the results. (A) Ku70 and Ku80, but not Ku70+Ku80, preferentially bind to the AP site. (B) GzmA cleaves Ku70 at Arg(301). Cleavage at this site separates the two Ku80 binding domains. The GzmA N-terminal Ku70 cleavage product (Ku701–300) binding and competition assays. (C) Ku701–115, but not Ku70115-609, bound to the AP/G substrate at a higher level than the C/G and U/G substrates.