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. 2014 Jan 23;9(1):e86470. doi: 10.1371/journal.pone.0086470

Figure 5. TAK1 knockdown reduces cell adhesion of FGFR3-associated bladder cancer.

Figure 5

FGFR3-positive MGHU3 (Y375C) bladder cancer cells were transfected with control or TAK1 siRNA and treated with FGFR3 inhibitor (PD173074) or ligand (FGF1). PD173074 was added 3 hours after transfection and remained present through the duration of the experiment. FGF1-treated cells were serum-starved overnight and FGF1 added 1 hour prior to plating on collagen. Forty-eight hours post-transfection, cells were counted and plated on collagen type IV-coated tissue culture plastic. Cells were allowed to adhere 3 hours and adherence determined by fluorescence intensity following uptake of Calcein-AM. Statistical analysis was performed using a t-test; (*) p<0.05; (***) p<0.001; (****) p<0.0001. Representative of five experiments.