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. 2014 Jan 16;63(2):585–595. doi: 10.2337/db13-0666

Figure 7.

Figure 7

SH2B1 promotes the Akt/FOXO1 pathway in islets. A: Pancreas sections were prepared from PKO and control mice fed an HFD (60% fat) for 10–12 weeks and immunostained with anti–phospho-Akt (pThr308) and anti-insulin antibodies. Akt phosphorylation was quantified using ImageJ software. a.u., arbitrary units. Control: n = 12; PKO: n = 10. B: Pancreatic sections were prepared from PKO (n = 11) and control (n = 11) mice fed an HFD for 10–12 weeks and coimmunostained with anti–phospho-FOXO1 (pSer256) and anti-insulin antibodies. C: Male mice (7–10 weeks) were fed an HFD (60% fat) for 5 weeks and fasted for 18–22 h. Islets were isolated and treated with insulin (100 nmol/L) for 15 min, and islet extracts were immunoblotted with anti–phospho-Akt (pSer473) or Akt, respectively. Phosphorylation of Akt was quantified and normalized to total Akt levels. SH2B1f/f (control): n = 8; PKO: n = 8. Data are means ± SE. *P < 0.05. Con, control.