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Demonstration of the restriction point in β-cells triggered to divide in vivo. A: Timed application of a mitogen (GKA) and a downstream inhibitor of its mitogenic activity (diazoxide) identifies the restriction point in β-cells in vivo after 7 to 8 h of mitogen activity. B: Model summarizing findings on β-cell kinetics in vivo. **P < 0.01. NS, not significant.
