Figure 6.

Drinking water pH affects the acquisition of microflora and leads to dysbiosis upon switching from AW to NW. Three- to four-week-old female NOD mice were purchased from JAX and continued on AW or switched to NW and subjected to FT as described for Fig. 5. These mice were killed at 8 weeks of age, and the DNA prepared from distal ileum samples was subjected to 16S rDNA–targeted pyrosequencing. The data were analyzed as described for Fig. 4, and the mean percentage values of bacterial sequences that were identified to species or phylum levels are shown. Upper pie charts represent all communities, and lower pie charts show communities other than Lactobacillus johnsonii. Percentage values of major non–L. johnsonii flora are shown.