Table 1. Primers used for amplification of NS4A (1–48).
Oligo | Sequence (5′-3′) |
1 | agcctgaccctgaatctgattaccgaaatgggtcgtctgccgacctttatgacccagaaagcacgtgatgcactggataatctgg |
2 | cagttctgcataccgctgaagccggtggtcgtgcatataatcatgcactgagcgaactgtaag |
3 | tgctttctgggtcataaaggtcggcagacgacccatttcggtaatcagattcagggtcaggct |
4 | gatccttacagttcgctcagtgcatgattatatgcacgaccaccggcttcagcggtatgcagaactgccagattatccagtgcatcac |
5 | ggaggaggatccgaaaacctgtattttcagagcctgaccctgaatctgattaccBamHI |
6 | ggaggagtcgacctcgagttacagttcgctcagtgcXhoI |
7 | gcgcgaattcagtacaagcttgctctgaacggEcoRI |
8 | gctagttattgctcagcgg (T7-Terminator-Primer; Novagen #69337-3) |
9 | ggaggaccgcggcggtagcctgaccctgaatctgaSacII |
10 | ggaggagtcgacctcgagttacagttcgctcagtgcSalI |
11 | gaaattaccgaagagggtcgtctgccgac |
12 | attcagggtcaggctctgaaaatacaggtt |
Note: The sequences of the restriction sites used for cloning are underlined, the name of the enzyme is given below.