Table 1. Primers used for amplification of NS4A (1–48).

Oligo	Sequence (5′-3′)
1	agcctgaccctgaatctgattaccgaaatgggtcgtctgccgacctttatgacccagaaagcacgtgatgcactggataatctgg
2	cagttctgcataccgctgaagccggtggtcgtgcatataatcatgcactgagcgaactgtaag
3	tgctttctgggtcataaaggtcggcagacgacccatttcggtaatcagattcagggtcaggct
4	gatccttacagttcgctcagtgcatgattatatgcacgaccaccggcttcagcggtatgcagaactgccagattatccagtgcatcac
5	ggaggaggatccgaaaacctgtattttcagagcctgaccctgaatctgattaccBamHI
6	ggaggagtcgacctcgagttacagttcgctcagtgcXhoI
7	gcgcgaattcagtacaagcttgctctgaacggEcoRI
8	gctagttattgctcagcgg (T7-Terminator-Primer; Novagen #69337-3)
9	ggaggaccgcggcggtagcctgaccctgaatctgaSacII
10	ggaggagtcgacctcgagttacagttcgctcagtgcSalI
11	gaaattaccgaagagggtcgtctgccgac
12	attcagggtcaggctctgaaaatacaggtt

Note: The sequences of the restriction sites used for cloning are underlined, the name of the enzyme is given below.