Table 1.
Size distribution of MSNPs in aqueous solutions.
Size (nm) |
Zeta Potential (mV) |
||||
---|---|---|---|---|---|
H2O | DMEM 10% serum |
BEGM | BEGM 2mg/ml BSA |
H2O/DMEM+serum/BEGM+BSA | |
MSNP-OH | 1966 | 408 | 1096 | 416 | −10.5/−6.8/−7.2 |
MSNP-Phosphate | 1975 | 306 | 867 | 439 | −8.9/−6.5/−5.8 |
MSNP-PEG | 2675 | 405 | 1215 | 542 | −10.4/−5.9/−4.5 |
MSNP-PEI 0.6 KD | 1689 | 415 | 1243 | 474 | +29.5/−7.8/−6.5 |
MSNP-PEI 1.2 KD | 1684 | 452 | 1298 | 502 | +38.7/−6.5/−7.1 |
MSNP-PEI 1.8 KD | 1053 | 510 | 1087 | 550 | +36.9/−5.4/−3.2 |
MSNP-PEI 10 KD | 614 | 702 | 917 | 684 | +34.1/−7.5/−6.9 |
MSNP-PEI 25 KD | 1473 | 1043 | 1544 | 825 | +30.8/−5.9/−4.0 |
Particle size and zeta potential in solution were measured by a ZetaSizer Nano (Malvern). DMEM = Complete Dulbecco’s Modified Eagle Media, which contains 10% fetal calf serum (FCS). BEGM = Bronchial Epithelial Growth Medium, which includes growth factors, cytokines, and supplements (no serum).