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. 2014 Jan 23;9(1):e86772. doi: 10.1371/journal.pone.0086772

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

DCs and auto breast tumor cells (from patient 1) were stained red and green by PKH26 and PKH67 and double positive cells were analyzed using FACS and confocal microscopy. A, FACS analysis of the non-adherent cells fraction from the total fusion products. DC phagocytosis of apoptotic tumor cells was calculated as the percentage of double-positive cells, and was approximately 42%. B, FACS analysis of DCs mixed with tumor cells. No significant phagocytosis was observed.