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. 2014 Jan 23;9(1):e86774. doi: 10.1371/journal.pone.0086774

Figure 1. Relative Mmp20 transcript expression levels in murine enamel organs from offspring of three different transgenic founder mice and controls.

Figure 1

qPCR was performed to quantify Mmp20 expression. Shown are Mmp20 expression results from enamel organs of adult incisors (A, B) that continuously erupt and that therefore contain all stages of enamel development. Also shown are Mmp20 expression levels in 5 day-old first molar enamel organs (C) that are predominantly in the secretory stage of enamel development. Incisors from mice transgenic for the Tg6 or Tg24 transgenes each expressed significantly more Mmp20 (P<0.05) whereas the Tg42 transgenic mice expressed significantly less Mmp20 (P<0.05) compared to endogenous levels when the transgenes were present in the Mmp20 null background (A). As expected, total expression of Mmp20 was highest when the transgenes were present in the Mmp20 wild-type background where combined expression of endogenous and transgenic Mmp20 was quantified (B). In contrast to incisors, transgene expression in the molars was highest in the Tg6 mice with Tg24 expressing mid-levels and, like the incisors, Tg42 was expressed at the lowest levels in molars (C). KO, Mmp20 knockout; WT, wild-type.