Figure 7. Neutralization of IL-17A and neutrophil depletion ameliorates enhanced weight loss.
Mice vaccinated intranasally with split influenza virus antigen (A/Uruguay/716/2007 (H3N2)) and CRX-601 were administered with anti-Ly-6G (IA8) or anti-IL-17A (100 µg) i.p. one day prior to 5LD50 challenge with influenza A/Hong Kong/1968 (H3N2) and then daily for a further 6 days post challenge. Control mice were vaccinated with split flu plus vehicle and treated with anti-Ly-6G or isotype control antibodies as indicated. Survival (A) and percentage weight loss (B) were examined following influenza virus challenge (10 mice/group). Black lines indicate weight loss in control (split-flu+vehicle) mice and red lines indicate weight loss in adjuvanted groups treated with the indicated antibody. At day 5 post influenza challenge, (C) viral titers in the lung relative to control mice were evaluated (Rat IgG2a) by RT-PCR. This data is representative of two independent experiments. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 are significantly different from control, Rat IgG2a unless otherwise stated (One-way ANOVA, Dunnet post-test).