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. 2014 Jan 23;9(1):e86838. doi: 10.1371/journal.pone.0086838

Figure 8. Autophagy inhibitor 3-MA blocked RIPostC autophagy-enhancing effect in ND mice.

Figure 8

(A) Western blot was performed to test autophagy extent after 3 hours of postreperfusion in the sham, IR, IR+RIPostC and IR+RIPostC +3MA group. (B–D) Bar graph showed the quantification of the immunoreactive band. In the ND groups, RIPostC upregulated autophagy, 3-MA reversed this upregulation. No similar changes were detected in the DM groups. Data were presented as means ± SEM, n = 5 per group. *P<0.05 vs. the sham group; # P<0.05 vs. the ND-IR group.