Table 2. Analysis of flaB DNA in ear, inoculation site (Inoc), heart base (HB), ventricular muscle (VM), quadriceps muscle (QM), tibiotarsus (Tt), and xenodiagnostic ticks (XenoDx) from mice treated with ceftriaxone commencing at 30 days after inoculation.
Interval | Mouse # | Ear | Inoc | HB | VM | QM | Tt | XenoDx |
2 months | 1 | −−− | −−− | −−− | −−− | −−− | +++ | 0/3* |
2 | +−− | −−− | ++− | −−− | −−− | −−− | 0/4 | |
3 | −−− | −−− | −−− | +++ | −−− | +−− | 0/2 | |
4 | +−− | −−− | −−− | −−− | −−− | +−− | 0/2 | |
4 months | 1 | −−− | −−− | −−− | −−− | −−− | −−− | ND |
2 | −−− | −−− | −−− | −−− | −−− | −−− | ND | |
3 | −−− | −−− | +−− | −−− | −−− | −−− | 0/3 | |
4 | −−− | −−− | −−− | −−− | −−− | ++− | ND | |
8 months | 1 | −−− | −−− | −−− | −−− | −−− | +−− | ND |
2 | −−− | −−− | −−− | −−− | −−− | −−− | 3/4 | |
3 | −−− | −−− | −−− | −−− | −−− | −−− | 0/2 | |
4 | −−− | −−− | −−− | −−− | −−− | −−− | ND | |
5 | −−− | −−− | −−− | +−− | −−− | −−− | 0/3 | |
6 | −−− | −−− | −−− | −−− | −−− | +−− | 1/6 | |
7 | −−− | −−− | −−− | −−− | −−− | −−− | 1/3 | |
8 | −−− | −−− | −−− | −−− | −−− | −−− | 2/2 | |
12 months | 1 | −−− | +−− | +++ | +++ | ++− | +−− | 0/10 |
2 | −−− | +−− | +++ | +−− | +++ | −−− | 1/10 | |
3 | ++− | −−− | +++ | +++ | ++− | +++ | 1/10 | |
4 | +−− | +++ | ++− | +−− | ++− | +−− | 1/10 | |
5 | −−− | +++ | +++ | +++ | +++ | −−− | 1/10 | |
6 | +++ | +++ | +++ | +−− | +++ | +++ | 0/10 | |
7 | −−− | +++ | +++ | +++ | +++ | +++ | 1/10 | |
8 | +++ | +++ | +++ | +++ | +++ | +++ | 1/10 |
Mice were tested at 2, 4, 8, or 12 months after completion of treatment. Three separate aliquots of each tissue homogenate were tested, and positive or negative PCR results for each aliquot are summarized for each tissue.
number of positive ticks/number of ticks tested.