Figure 3. Over-expression of TRESK subunits increases the background K⁺ currents in TG neurons.

A, Upper panel: the voltage protocol used to minimize transient voltage-gated K⁺, Na⁺ and Ca²⁺ currents. Middle and lower panels: representative current traces from TG neurons expressing mCherry (the control group) and mCherry-TRESK (the TRESK group) before and after the application of 30 µM lamotrigine, respectively. B, Mean total outward current density (measured at the end of the depolarizing step) in TG neurons expressing mCherry and mCherry-TRESK, respectively (*** p<0.001, two-tailed t-test, n = 22 and 21 cells in each group, respectively). C, The percentage of outward current (measured at the end of the depolarizing step) inhibited by lamotrigine in the control and TRESK groups, respectively (* p<0.05, two-tailed t-test, n = 17 and 11 cells in each group, respectively).