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. 2014 Jan 23;9(1):e87203. doi: 10.1371/journal.pone.0087203

Figure 1. Trichostatin A induces DNA damage.

Figure 1

(A) HEK293T ELG1-LUC cells were treated with 0.75 µM TSA or 50 µM SAHA for 48 hr. LUC activity was measured using One-Glo. The graph represents average LUC activity ± SD. Significance was calculated using a two-tailed t-test. (B) HEK293T cells were treated with 50 µM TSA for 24 hr. Using the indicated antibodies proteins levels were visualized by Western blot analysis from either the chromatin-bound fraction or total cell lysate. (C) HEK293T cells were treated with 100 uM TSA for 24 hours and then Pulsed-field gel electrophoresis was performed.