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. 2014 Jan 23;9(1):e87373. doi: 10.1371/journal.pone.0087373

Figure 2. Effect of vinculin and Rab5 expression on cellular uptake.

Figure 2

(A–E) HA-vinculin-expressing Cos-7 cells were incubated with various markers of uptake at 37°C for 2 h. Uptake of pHrodo red-labeled S. aureus BioParticles increased in HA-vinculin-expressing cells, but the uptake of transferrin Alexa 555, albumin Alexa 555, Lucifer yellow and FM 4-64 did not increase. Error bar: n = 3−6±SE, **p<0.01. (F–H) HA-Rab5 (S34N)-, HA-Rab5 (WT)-, and HA-Rab5 (Q79L)-expressing Cos-7 cells were incubated with various uptake indicators at 37°C for 2 h. Uptake of pHrodo red-labeled S. aureus, transferrin Alexa 555, albumin Alexa 555, Lucifer yellow, and FM 4-64 increased in HA-Rab5 (WT)- and HA-Rab5 (Q79L)-expressing cells, whereas uptake of each of the indicators was decreased in HA-Rab5 (S34N)-expressing cells. Error bar: n = 3–6±SE, *p<0.05, **p<0.01.