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. 2014 Jan 23;9(1):e87373. doi: 10.1371/journal.pone.0087373

Figure 8. Effect of vinculin and Rab5 knockdown on S. aureus-induced IL-6 expression.

Figure 8

(A and B) S. aureus was added to the medium of HeLa cells with vinculin (A) or Rab5 (B) knockdown and incubated for 60 min at 37°C. The cell lysates were assayed with anti-JNK, anti-phosphor-JNK, anti-ERK, anti-phosphor-Erk, anti-p38, and anti-phosphor-p38 antibodies by western blotting. (C and D) S. aureus was added to the medium of HeLa cells with vinculin or Rab5 knockdown and incubated for 16 h. The cell lysate (C) and conditioned medium (D) were assayed with anti-IL-6 antibody by western blotting. GAPDH and albumin were internal controls.