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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Clin Cancer Res. 2009 Aug 11;15(16):5073–5081. doi: 10.1158/1078-0432.CCR-09-0092

Fig. 3.

Fig. 3

Anti-miR-221 increases the susceptibility to apoptosis in SNU449 cells grown in nonadherent conditions. Bmf is released during nonadherent growth and triggers apoptosis. Following cell plate detachment, SNU449 cells progressively dye. Anti-miR-221 transfection in this experimental setting is associated with an increased number of dead cells (A), which is likely related with apoptosis as shown by increased Bmf and caspase-3 levels (B) at 24 h. NC, negative control. In nonadherent conditions (C), Bmf silencing (by Bmf siRNA) following anti-miR-221 (AM-221) transfection is associated with a decrease of caspase-3 activation (AM-221 + and Bmf siRNA +) when compared with anti-miR-221-transfected cells (AM-221 + and scramble +). Ratio between Bmf or cleaved caspase-3 (17 kDa isoform) and β-actin corresponding bands (absorbance).