Figure 3. FLH is more immunogenic than CCH and KLH.

(A). Humoral immune response against hemocyanins. Groups of 3 mice were immunized ip with 200 µg of FLH or CCH and KLH in PBS, and the immunization was repeated 15 days later. Ten days later, specific antibody titers were determined by ELISA from serially diluted sera. The mouse IgG antibodies were revealed using goat anti-mouse IgG ALP-conjugated serum; subsequently, pNPP was added. The reaction was read at 405 nm. Data are shown as mean + SEM of n = 3 mice per group and are representative of two independent experiments, *p<0.05 between FLH versus KLH and CCH. (B). Determination of the carrier property of FLH. The hemocyanins were coupled to DNFB; subsequently, groups of 3 C57BL/6 or BALB/c mice were immunized ip with 300 µg of each conjugate with Freund's adjuvants as a repository. Ten days after, the titers against DNFB were determined by ELISA. FLH versus CCH and KLH p not significant.