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. 2014 Jan 23;9(1):e87240. doi: 10.1371/journal.pone.0087240

Figure 5. Immunotherapeutic effect of FLH in the B16F10 mouse melanoma model does not require priming.

Figure 5

(A). Schedule of the bioassay. (B). Effect of FLH on melanoma growth. Tumor growth was followed every 3 days, and each group contained 7 mice; ***p<0.001 between FLH and PBS. (C). Effect of FLH on the survival of mice. The log-rank test was used to compare the curves; *p<0.05 between FLH and PBS. (D). Kinetics of tumor appearance in mice. The percentages of mice bearing tumors were determined visually and by palpation. (E). Tumor-infiltrating immune cells. The quantitative analysis of tumor infiltration by CD8/CD3+, CD11c+, NK1.1+, F4/80+, CD4/CD3+ and CD19+-positive cells in single-cell suspensions derived from B16F10 tumors was performed on day 18 of the bioassay. The results are expressed as the percentages of total viable immune cells. Each group consisted of 3–4 mice; *p<0.05. (F). IFN-γ measurements from mouse sera were obtained on day 18 of the bioassay (same mice and day as the experiment shown in E); *p<0.05 FLH versus PBS.