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. 2014 Jan 23;9(1):e87240. doi: 10.1371/journal.pone.0087240

Figure 6. Effect of periodate treatment on anti-tumor properties of FLH in the B16F10 mouse melanoma model.

Figure 6

(A). Schedule of the bioassay. Groups of 5–7 mice were sc primed with FLH or Ox-FLH (400 µg/100 µl PBS). After 19 days, the animals were challenged sc with 1.5×105 B16F10 melanoma cells, and they then received intralesional injections of 100 µg of each hemocyanin for 6 consecutive days. (B). Effect of Ox-FLH on melanoma growth. Tumor growth was followed every 3 days until day 25, and each group consisted of 5 mice; ***p<0.001 between FLH and Ox-FLH versus PBS. (C). Effect of Ox-FLH on the survival of mice. Mouse survival was recorded until day 100 of the bioassay. The survival curves were compared using the log-rank test; ***p<0.001 FLH versus PBS, *p<0.05 between Ox-FLH versus PBS. (D). Ox-FLH was more immunogenic than its native counterpart; *p<0.05 between FLH and Ox-FLH.