Skip to main content
. 2014 Jan 23;9(1):e87521. doi: 10.1371/journal.pone.0087521

Figure 1. A point mutation in Hsp104 destabilizes [PSI+].

Figure 1

(A–B) Cells containing hsp104-V426I or HSP104 were plated onto solid rich medium (YPD) to illustrate the destabilizing effect that this mutation has on the [PSI+] phenotype. (A) In the presence of hsp104-V426I, [PSI+] is lost in a fraction of the buds, generating sectors of [psi−] cells (phenotypically red) in the [PSI+] colony. (B) Cells expressing hsp104-V426I lose the [PSI+] prion more frequently than HSP104 cells. (C) The copper-inducible fluorescent protein, Sup35NM-GFP, was ectopically expressed in hsp104-V426I [PSI+] cells along with wild type [PSI+] and [psi−] cells. Fluorescence imaging was performed on an Olympus confocal microscope and representative images are shown.