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. 2014 Jan 23;9(1):e87521. doi: 10.1371/journal.pone.0087521

Figure 2. Hsp104 M-domain mutants affect ATPase activity.

Figure 2

The ATPase activity of recombinant wild type (WT) Hsp104, Hsp104-V426I, Hsp104-V426C, Hsp104-D434A, Hsp104-K480C, and Hsp104-Y507D was measured by the Malachite Green assay after incubation of 2 µg of protein with 5 mM ATP at 37°C either in the absence (black) or presence (grey) of 0.25 mg/mL β-casein. The amount of free inorganic phosphate in each sample was calculated from analysis of phosphate standards. For each protein, the average initial rate of ATP hydrolysis is plotted. Each protein was assayed in quadruplicate from two separate purification preparations and the error bars reflect standard deviation between the samples.