Figure 4. Hsp104-K480C and Hsp104-Y507D are toxic at high temperatures.

hsp104Δ strains expressing wild type HSP104, hsp104-V426I, hsp104-V426C, hsp104-D434A, hsp104-K480C, or hsp104-Y507D from a HIS3-containing plasmid, were plated on solid medium lacking histidine and grown at 25, 30, or 37°C to assess temperature-dependent growth defects, as compared to an empty vector control (Vector). Dashed lines represent different parts of the same plate that have been cropped for clarity. These spottings are representative of three independent experiments.