Table 1. Summary of biochemical and biological effects of the Hsp104 M-domain mutants.
| WT | V426I | V426C | D434A | K480C | Y507D | |
| ATPase Activity | + | + | − | − | ++ | ++ |
| Hexamerization | + | + | − | − | + | + |
| Temp. Sensitivity | + | + | + | + | ++ | ++ |
| Thermotolerance | + | + | + | − | +/− | +/− |
| Luciferase Refolding | + | +/− | +/− | − | +/− | +/− |
| Strong [ PSI +] 2n | + | +/− | +/− | − | +/− | +/− |
| Weak [ PSI +] 2n | + | + | +/− | − | +/− | +/− |
| Strong [ PSI +] n | + | +/− | +/− | − | +/− | NT |
| Weak [ PSI +] n | + | − | +/− | − | − | NT |
| s.d. low [ RNQ +] | + | + | − | − | +/− | − |
| s.d. med [ RNQ +] | + | − | − | − | − | − |
| s.d. high [ RNQ +] | + | + | +/− | − | +/− | − |
| s.d. very high [ RNQ +] | + | + | − | − | − | − |
| m.d. high [ RNQ +] | + | + | +/− | − | +/− | + |
Effects of Hsp104 mutations were characterized as follows for the indicated properties and prion propagation, as compared to wild type (WT) Hsp104: (+) comparable to WT, (+/−) some defect, (−) abolished/cured, or (++) enhanced activity or sensitivity. NT: not tested, 2n: yeast diploids, n: yeast haploids.