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. 2014 Jan 22;7:42. doi: 10.1186/1756-3305-7-42

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Copyright © 2014 Antunes et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Production of recombinant proteins and antibodies. (A) Ten micrograms of recombinant proteins TROSPA, SILK, Subolesin (SUB) and thioredoxin (TRX; negative control) were loaded onto an SDS-PAGE gel under reducing conditions and visualized by Coomassie brilliant blue staining. (B) Western blot analysis of the recombinant proteins using purified IgGs produced in rabbits immunized with these proteins. Hybridization signals were developed with an anti-rabbit IgG HRP conjugate and TMB substrate for HRP. Arrows indicate the position of recombinant proteins. Abbreviation: MW, molecular weight.