Figure 4.
Effect of antibodies on tick weight. (A) Ticks capillary-fed on uninfected blood without (Blood) and with preimmune and anti-tick protein IgGs (TROSPA, SILK and Subolesin). (B) Ticks capillary-fed on B. bigemina-infected blood without (Blood) and with preimmune and anti-tick protein IgGs. (C) Ticks capillary fed on A. marginale-infected blood without (Blood) and with preimmune and anti-tick protein IgGs. Ticks (N = 15) were weighed before and after capillary feeding, the tick weight increase calculated as final minus initial tick weight, expressed as Ave + S.D. (mg/tick) and compared between the group with preimmune antibodies and the other groups by Student’s t-test (*P < 0.05).